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EC Number Cloned (Commentary)
Show all pathways known for 4.2.1.82Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.82heterologously expressed in Escherichia coli
Show all pathways known for 4.2.1.82Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.82PCR product cloned into pET19b. The resulting plasmid harvested from Escherichia coli JM109, sequenced, and transformed into Escherichia coli Rosetta(DE3)-pLysS expression strain. In-frame deletion mutant amplified and ligated into pTA131, sequenced, and transformed in Haloferax volcanii H26 DELTApyrE2
Show all pathways known for 4.2.1.82Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.82xylXABCD, xylXAD, xylXD, xylD or xylX ligated into the vector pJTmcs using the KpnI, XbaI, and XmaJI restriction sites, under the control of the constitutive tac promoter, expressed in Pseudomonas putida S12
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