EC Number   |
|---|
   3.6.5.3 | - |
| 3.6.5.3 | comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. I15V. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a increased efficiency in protein synthesis, which is accompanied by an reduced affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an decreased efficiency in the intrinsic GTPase activity |
| 3.6.5.3 | comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. V15I. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a reduced efficiency in protein synthesis, which is accompanied by an increased affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an increased efficiency in the intrinsic GTPase activity |
| 3.6.5.3 | eEF2 in vitro translation by rabbit reticulocyte lysate |
| 3.6.5.3 | expressed in Escherichia coli |
| 3.6.5.3 | expression in Escherichia coli |
| 3.6.5.3 | expression in Escherichia coli BL21 |
| 3.6.5.3 | expression in Escherichia coli using the pT7-7 expression vector |
| 3.6.5.3 | for expression in Escherichia coli cells |
| 3.6.5.3 | full-length enzyme or C2 subdomain of ymIF2 expressed in Escherichia coli. The full-length ymIF2 can substitute for Escherichia coli IF2 in the formation of a functional initiation complex on 70S Escherichia coli ribosomes capable of forming the first peptide bond |
