EC Number   |
|---|
  3.5.1.136 | amplified by polymerase chain reaction, cloned into expression vector pET15b, and expressed in E. coli BL21-codonPlus (DE3)-RIL |
| 3.5.1.136 | expression in Bacillus subtilis |
| 3.5.1.136 | expression in Bacillus subtilis WB600 is better than expression in Escherichia coli BL21(DE3) |
| 3.5.1.136 | expression in Escherichia coli |
| 3.5.1.136 | no expression in the soluble fraction of Escherichia coli harboring the expression plasmid. Insertion of the target genes into the chromosome of Escherichia coli yields the soluble recombinant protein |
| 3.5.1.136 | overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys and successfully refolded in vitro |
