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Cloned (Commentary)
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3.4.23.B2
cloned directly from proviral DNA of the infectious viral stock SIVmac251-32H(11/88 pool). SIVmac251-32H proteinase and ist flanking pol sequences are expressed in Escherichia coli as a fusion protein with most of the T7 bacteriophage gene 10 protein. The expressed protein forms cytoplasmic inclusion bodies which are solubilized in 8 M urea, and the recombinant enzyme is refolded, yielding active self-processing enzyme
647807
3.4.23.B2
expressed in Escherichia coli with a recombinant expression system
647814
3.4.23.B2
expression in Escherichia coli
647806
3.4.23.B2
expression in Escherichia coli, expression as N-terminally capped, biotin-labeled precursor
668793
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