EC Number |
---|
3.2.1.68 | - |
3.2.1.68 | cloned to an expression vector with a T7lac promoter. Both wild-type and His-tagged isoamylases are expressed in Escherichia coli |
3.2.1.68 | cloning of cDNAs of different isoforms, Psisa1-Psisa3, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparisons, overview |
3.2.1.68 | coexpression of wild-type His-tagged ISA1 and ISA2, and of mutant D367A ISA1 with wild-type ISA2, in Escherichia coli strain BL21(DE3) |
3.2.1.68 | DNA and amino acid sequence determination and analysis, phylogenetic tree |
3.2.1.68 | DNA and amino acid sequence determination and analysis, sequence comparisons |
3.2.1.68 | expressed in Escherichia coli |
3.2.1.68 | expressed in Escherichia coli BL21 cells |
3.2.1.68 | expressed in Escherichia coli BL21(DE3) cells |
3.2.1.68 | expressed in Escherichia coli BL21(DE3) Star cells |