EC Number |
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3.2.1.3 | - |
3.2.1.3 | cloning of gene glaA encoding glucoamylase G1 of Aspergillus tubingensis strains 643.92 and 127.49 reveals high identity with the gene/enzyme from Aspergillus niger strain DSM 823, DNA and amino acid sequence determination and analysis, phylogenetic analysis |
3.2.1.3 | construction of combinatorial library of starch-binding domain of the enzyme, expression in Escherichia coli strain DH5alpha, screening of clones with enhanced activity by yeast display method using Saccharomyces cerevisiae strain MT8-1, overview |
3.2.1.3 | DNA and amino acid sequence determination and analysis, constitutive overexpression of the His-tagged enzyme in Escherichia coli |
3.2.1.3 | DNA and amino acid sequence determination and analysis, sequence comparisons |
3.2.1.3 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |
3.2.1.3 | DNA and amino acid sequence determination and anaylsis of wild-type and mutant enzymes, expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain C468 using vector YEpPM18 |
3.2.1.3 | DNA and amino acid sequence determination, analysis and comparisons |
3.2.1.3 | expressed in Saccharomyces cerevisiae |
3.2.1.3 | expressed in transgenic rice seeds |