EC Number   |
|---|
    3.2.1.26 | - |
| 3.2.1.26 | 48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32 |
| 3.2.1.26 | CIN1, functional expression of the enzyme in roots of Arabidopsis thaliana under control of the root-specific pyk10 promoter |
| 3.2.1.26 | CIN12 is inserted into the expression vector pET_ZZ_1a, transformed into Escherichia coli BL21 DE3 and overexpressed |
| 3.2.1.26 | cloned into a pET29a-derived vector with an N-terminal His6 tag. Likewise, the truncated InvB covering residues Thr9-Ser457 is constructed for crystallization. Both the wild-type and mutant proteins are overexpressed in Escherichia coli strain BL21(DE3). Selenomethionine (SeMet)-substituted full-length and truncated InvB are overexpressed in Escherichia coli B834 (DE3) |
| 3.2.1.26 | DNA and amino acid sequence determination, expression of His-tagged wild-type and selenomethionine-labeled enzyme in Escherichia coli strain BL21(DE3) |
| 3.2.1.26 | DNA and amino acid sequence determination, map-based cloning of soluble isozyme |
| 3.2.1.26 | Escherichia coli BL21 (DE3) with invbpetK plasmid, 37°C, 2TY medium |
| 3.2.1.26 | expressed in Arabidopsis thaliana |
| 3.2.1.26 | expressed in Escherichia coli and in an invertase-negative strain of Saccharomyces cerevisiae |
