EC Number   |
|---|
    3.2.1.141 | - |
| 3.2.1.141 | DNA construct pRBMhMTH expressed in Escherichia coli BL21(DE3)pLysS |
| 3.2.1.141 | Escherichia coli BL21(DE3)pLysS, LB medium, vector pRSET-B |
| 3.2.1.141 | Escherichia coli Rosetta (DE3) with mutated vector pET-15b-deltaH-treZ, Terrific broth, 37°C |
| 3.2.1.141 | expressed in Escherichia coli BL21 (DE3) pLysS cells |
| 3.2.1.141 | expression in Escherichia coli |
| 3.2.1.141 | expression of wild-type and mutant enzymes in Escherichia coli |
| 3.2.1.141 | gene ST0926, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes |
| 3.2.1.141 | gene ST0927, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes |
| 3.2.1.141 | gene treZ, codon-optimized synthetic gene, recombinant expression of SUMO-fused enzyme in Escherichi coli strains BL21 (DE3) and Origami (DE3), subcloning in Escherichia coli strain JM109, host strain and induction strategy optimization for high-efficiency expression, method reaching a maximum of 204.6 U/ml for the soluble enzyme, detailed overview |
