EC Number |
---|
3.1.7.2 | cloning of relSeq fragments and the expression is regulated using pBAD vectors |
3.1.7.2 | expression of fragment proteins in Escherichia coli BL21(DE3) |
3.1.7.2 | preparation of mutants with null alleles at both, spoT and relA |
3.1.7.2 | recombinant expression of full-length enzyme in Escherichia coli and in HEK-293T cells |
3.1.7.2 | recombinant expression of full-length enzyme in Escherichia coli and in S2 cells |
3.1.7.2 | spotT gene |
3.1.7.2 | the pET22b expression system is used |