EC Number   |
|---|
   3.1.4.39 | - |
| 3.1.4.39 | alpha isoform of ATX, overexpression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 3.1.4.39 | atx-/-, atx+/-, and atx+/+ uncover the physiological role of ATX and to identify the endogenous product of ATX, ATX gene is targeted with an ATXgene-targeting vector. Plasma lysoPLD activity, plasma lysophosphatidic acid concentrations and plasma ATX protein level in atx+/-mice are about half the values found in atx+/+ mice. Embryos, early blood vessels appear to form properly, but fail to develop into mature vessels. ATX-null mice are lethal around embryonic day 10.5. Despite the reduced LPA level, heterozygous mice appear phenotypically normal. Viability and fecundity are similar to those in wild-type mice. Morphologies of atx+/+ and atx-/-embryo proper at E8.5, E9.5, and E10.5. Defects in vascular remodeling in atx-/-embryos shown. ATX and LPA stabilize preformed vessels and prevent endothelial disassembly in allantois explants |
| 3.1.4.39 | cloned from 129S6/Sv mouse brain, expression in COS-7 cells |
| 3.1.4.39 | co-transfection of MBEC4 cells with 2 plasmids, mlysoPLDpCAGGS26 and pSV2-neo, screening by Western blotting and immunocytochemistry with an anti-ATX mAb (4F1)14 and by measuring the lysoPLD activities of the culture media |
| 3.1.4.39 | COS-7 cells (ATCC: CRL-1651) and HEK 293 cells. To gain further insight into the possible functional role of ATX in the brain, analysis of its cellular expression in the brain under physiological conditions and following trauma. Preparation and purification of oligodendrocyte precursor cells: Primary mixed glial cultures are prepared from the cerebral hemispheres of P0P2 Sprague-Dawley rat pups from which the meninges has been stripped |
| 3.1.4.39 | expressed in B-103 cells |
| 3.1.4.39 | expressed in COS-7 cells |
| 3.1.4.39 | expressed in COS-7 cells (ATCC: CRL-1651) and HEK 293 cells. Compared distribution of overexpressed ATX in COS-7 and HEK293 cells |
| 3.1.4.39 | expressed in Escherichia coli BL21 and Escherichia coli TOP10 strain. Construction of an expression system with Escherichia coli and purification methods for the active recombinant molecule of human ATX |
