EC Number |
---|
3.1.3.8 | - |
3.1.3.8 | cloning from genomic DNA, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative realtime PCR expression analysis |
3.1.3.8 | concerted action of endogenous phytase, EC 3.1.3.26, and heterologous phytase from Aspergillus niger, EC 3.1.3.8, on phytic acid degradation in seed of transgenic Triticum aestivum L. |
3.1.3.8 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the His-tagged full-length enzyme and its N- and C-terminal domains in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |
3.1.3.8 | DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
3.1.3.8 | DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
3.1.3.8 | engineering of Escherichia coli appA phytase gene into the chloroplast genome of the model microalga, Chlamydomonas reinhardtii strain CC-125, and isolation of homoplasmic plastid transformants. Catalytic activity of the recombinant AppA can be directly detected in the whole-cell lysate with 10 phytase units per gram dry matter at pH 4.5 and 37°C, method optimization, overview |
3.1.3.8 | expreession in Solanum tuberosum under control of sweet potato sporamin promoter |
3.1.3.8 | expressed in Aspergillus awamori strains pGP209 and pGF11 |
3.1.3.8 | expressed in Aspergillus niger |