EC Number   |
|---|
   3.1.3.12 | - |
| 3.1.3.12 | cloned as an operon and expressed in Escherichia coli M15(pREP4) |
| 3.1.3.12 | cloning of wild-type and mutant tagged enzyme with an N-terminal fusion peptide (MGSSH6SSGRENLYFQGH), recombinant expression in Escherichia coli |
| 3.1.3.12 | commentary |
| 3.1.3.12 | construction of homozygous and heterozygous deletion mutants, the homozygous deletion mutants has little or no enzyme activity, the complete inactivation of enzyme results in 50fold hyperaccumulation of trehalose 6-phosphate, thermosensitivity, and rapid death of the cells after a few hours at 44°C |
| 3.1.3.12 | DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) and release into the soluble fraction with a natively folded conformation |
| 3.1.3.12 | expressed in Arabidopsis thaliana |
| 3.1.3.12 | expressed in Escherichia coli |
| 3.1.3.12 | expressed in Escherichia coli as a His-tagged fusion protein |
| 3.1.3.12 | expressed in Escherichia coli BL21 cells |
