EC Number   |
|---|
    3.1.1.3 | - |
| 3.1.1.3 | 2 orfs: CpLIP1 and CpLIP2, DNA and amino acid sequences determination and analysis, expression in Saccharomyces cerevisiae of both forms as C-terminally His-tagged proteins, only CpLIP2 encodes for an active enzyme |
| 3.1.1.3 | amino acid determinatioin of the purified enzyme, high-level expression in Pichia pastoris GS115, subcloning in Escherichia coli DH5alpha |
| 3.1.1.3 | ATGL gene, DNA and amino acid sequence determination and analysis, phylogenetic and tissue expression analysis |
| 3.1.1.3 | ATGL gene, DNA and amino acid sequence determination and analysis, tissue expression analysis |
| 3.1.1.3 | BTID-B, DNA sequence determination and analysis, expression in Escherichia coli |
| 3.1.1.3 | CalA, subcloning in Escherichia coli strain DH5alpha, functional expression in Pichia pastoris strain X 33, development of a fed-batch process and a semi-continuous process, overview |
| 3.1.1.3 | cloned and overexpressed in Escherichia coli cells |
| 3.1.1.3 | cloning of lipase gene lipA and its activator gene lipB, immediately downstream of lipA, DNA and amino acid sequences determination and analysis, overexpression of the insoluble protein in Escherichia coli BL21(DE3) with coexpression of lipB resulting in an active enzyme, enzyme expressed without lipB is not active |
| 3.1.1.3 | co-expression of CS-2 lipase and its cognate foldase in Escherichia coli BL21(DE3) cells |
