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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.8.5.2- 748677
Display the word mapDisplay the reaction diagram Show all sequences 2.8.5.2the heterodimeric cytochrome complex SoxXA is produced in Escherichia coli. The soxX and soxA genes, separated by two genes in the sox gene cluster are fused with ribosome binding sites optimal for Escherichia coli and combined to give soxXA in pRD133.27. The cytochrome complex SoxXA is produced in Escherichia coli M15 containing pRD133.27, pREP4 encoding the Lac repressor and plasmid pEC86, carrying essential cytochrome c maturation genes. This cytochrome complex is equally catalytically active with respect to rates and reactivity with different sulfur substrates in the reconstituted sulfur-oxidizing enzyme system using homogeneous Sox-proteins of Paracoccus pantotrophus 747161
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