EC Number   |
|---|
   2.7.7.48 | - |
| 2.7.7.48 | a codon-optimized cDNA sequence of ZIKV NS5 (amino acids 2522 to 3423; strain H/PF/2013) is synthesized de novo. The ZIKV NS5 polymerase portion is PCR amplified and cloned into a pMal-c5X vector under tac promoter control. Expression in Escherichia coli |
| 2.7.7.48 | a hexahistidine affinity-tagged NS5B fusion protein is expressed with recombinant baculoviruses in insect cells |
| 2.7.7.48 | bacterial production of the recombinant DENV-2 RdRp was difficult due to its low expression and solubility levels, recombinant expression of three different forms of DENV-2 RdRp as either N- or C-terminally His-tagged fusions, or without tag in Escherichia coli strains Rosetta (DE3) and BL21 (DE3). The presence of both the N- and C-terminal His-tag has a deleterious effect on polymerase activity, overview |
| 2.7.7.48 | C-terminal hydrophobic domain deletion mutant expressed in Escherichia coli |
| 2.7.7.48 | cloned and expressed in Escherichia coli with a C-terminal hexahistidine tag |
| 2.7.7.48 | co-expression of SARS-CoV-2 RNA-dependent RNA polymerase subunits with chaperones gives soluble expression in Escherichia coli |
| 2.7.7.48 | DNA and amino acid sequence determination and analysis, phylogenetic analysis based on the sequence of the RdRp whole domains, overview |
| 2.7.7.48 | DNA and amino acid sequence determination and analysis, sequence comparisons, NS5B genotyping, enzyme activities, and activity with ribavirin, overview. Expression of His-tagged enzyme variants in Escherichia coli |
| 2.7.7.48 | DNA sequence determination and analysis of hundreds of intronic regions encoding three or more RNA-dependent RNA polymerase-dependent sRNAs that are covered by dsRNA-seq (double-stranded RNA sequencing) reads, indicating that the intron-derived sRNAs are indeed generate from long double-stranded RNA precursors |
