EC Number |
---|
2.7.2.3 | - |
2.7.2.3 | 129S6/SvEvTac genomic DNA fragments inserted into vector pUCBM21 |
2.7.2.3 | 1872-bp Pgk1/EGFP DNA fragment excised by double digestion of MluI and DraIII, consisting of 519 bp of the Pgk1 promoter, 723 bp of the EGFP cDNA and 224 bp of the bovine growth hormone (BGH) poly-A tail from the vector pPgk1/EGFP. Microinjection into the pronucleus of in vitro-fertilized FVB/NCrl mouse eggs and re-implanted into pseudopregnant ICR females to generate transgenic mice. CHO-K1 cells transfected with pPgk1/EGFP |
2.7.2.3 | 3'UTR of PGK2 subcloned into the three regions F1, F2 and F3 |
2.7.2.3 | a translational fusion of PGK and green fluorescent protein is used to transform tobacco BY-2 cells resulting in green fluorescent protein locating to the cell nuclei |
2.7.2.3 | cloned from genetic library, DNA sequence determination and analysis, expression in Escherichia coli as glutathione-S-transferase-fusion protein, which is subsequently cleaved off with thrombin |
2.7.2.3 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM 83 |
2.7.2.3 | DNA and amino sequence analysis, phylogenetic analysis, sequence comparisons, expression in Escherichia coli strain JM109 |
2.7.2.3 | DNA sequence determination and expression in Escherichia coli BL21(DE3) |
2.7.2.3 | expressed in Escherichia coli |