EC Number |
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2.7.1.127 | a cDNA encoding InsP3 3-kinase B is cloned from a thymus cDNA library and expressed in Escherichia coli, sequence of the 673 amino acids protein |
2.7.1.127 | cDNA encoding InsP3 3-kinase B is cloned from a hippocampus cDNA library and expressed in Escherichia coli, amino acid sequence |
2.7.1.127 | cDNAs encoding enzyme isoforms A from brain and B are cloned and overexpressed in B31 cells, a rat-1 fibroblast cell line expressing pp60v-src |
2.7.1.127 | Cloning and expression of isoform A and of a fragment comprising the catalytic calmodulin binding domain (amino acids 165-462) in Escherichia coli BL21(DE3). |
2.7.1.127 | Cloning and expression of isoform A and of an enzyme fragment comprising the catalytic calmodulin binding domain in Escherichia coli BL21(DE3). Creating of a point mutant by using PCR-based site-directed QuikChange mutagenesis and expression in Escherichia coli. |
2.7.1.127 | Cloning and expression of isoform B and of a fragment comprising the catalytic calmodulin binding domain (amino acids 165-462) in Escherichia coli BL21(DE3). |
2.7.1.127 | cloning and expression of the isoenzymes A, B and C in COS-7 cells, the 3 isoenzymes A, B and C can be distinguished by their localization on transfection in COS-7 cells |
2.7.1.127 | cloning of a full-length cDNA encoding IP3K isoform C from tongue epithelium, cloning of an enzymatically active and Ca2+/calmodulin-regulated fragment of isoform C and expression in Escherichia coli BL21(DE3)pRIL |
2.7.1.127 | cloning of isozyme IP3K-A from erythrocytes and expression of a fragment comprising the catalytic domain and the CaM binding domain of the wild-type and mutant enzyme, respectively, in Escherichia coli strain BL21(DE3) |
2.7.1.127 | Cloning, Expression, and Purification of RnIP3K-C isoform and of two different recombinant fragments IP3K-C, one comprising the catalytic domain and the calmodulin binding domain, the other comprising only the catalytic domain, are expressed in Escherichi coli BL21(DE3) cells. |