EC Number |
---|
2.7.1.105 | - |
2.7.1.105 | both parent and double mutant (K472A/K473A) constructs are transformed into the BL21 strain of Escherichia coli, overexpression of PFKFB3 causes no change in glucose metabolism but rather a marked increase in cell proliferation |
2.7.1.105 | DNA and amino acid sequence determination and analysis |
2.7.1.105 | DNA and amino acid sequence determination and analysis, phylogenetic analysis of the 6-phosphofructo-2-kinase and the fructose-2,6-bisphosphatase domains, expression of His-tagged wild-type and mutant isozymes Tb1, Tb2, and Tb4 in Escherichia coli, poor expression levels and mostly inactive and unstable isozymes, e.g. recombinant Tb2 is inactive |
2.7.1.105 | expressed in Escherichia coli |
2.7.1.105 | expressed in Escherichia coli, overexpression under the control of the phosphoenolpyruvate carboxykinase promoter in Mus musculus, microinjection of fertilized eggs, C57BL56/SJL receptor females of Mus musculus implanted with microinjected oocytes |
2.7.1.105 | expressed in FVB mice, standard embryo microinjection procedures |
2.7.1.105 | expression in Escherichia coli |
2.7.1.105 | expression in Saccharomyces cerevisiae |
2.7.1.105 | expression in Spodoptera frugiperda cells infected with recombinant baculovirus and Escherichia coli |