EC Number   |
|---|
    2.5.1.1 | - |
| 2.5.1.1 | a homomeric geranyl-diphosphate synthase from Camptotheca acuminata, a camptothecin-producing plant, is obtained from 5'- and 3'-rapid amplification of cDNA ends and subsequent overlap extension and convenient PCR amplifications. The truncate enzyme (tCaGPPS) is introduced to replace ispA of pBbA5c-MevT(CO)-MBIS(CO, ispA), a de novo biosynthetic construct for farnesyl diphosphate generation, and overexpressed in Escherichia coli together with the truncated geraniol synthase-encoding gene (tCaGES) from Camptotheca acuminata, to confirm geranyl-diphosphate synthase-catalyzed reaction in vivo. The tCaGPPS and tCaGES genes with different copy numbers are introduced into Escherichia coli to balance their catalytic potential for high-yield geraniol production. A 1.6fold increase of geraniol production is obtained when four copies of tCaGPPS and one copy of tCaGES are introduced into Escherichia coli. The following fermentation conditions optimization, including removal of organic layers and addition of new n-decane, leads to a 74.6 mg/l of geraniol production |
| 2.5.1.1 | expresion in Escherichia coli after truncation of the signal sequence at the 5'-end of the coding region |
| 2.5.1.1 | expressed in Camelina sativa |
| 2.5.1.1 | expressed in Escherichia coli |
| 2.5.1.1 | expressed in Escherichia coli strain C41 |
| 2.5.1.1 | expression in Escherichia coli |
| 2.5.1.1 | expression in Escherichia coli and in Picea abies embryogenic tissue |
| 2.5.1.1 | the enzyme with an N-terminal His6 tag is transformed into Escherichia coli BL21 (DE3) cells |
