EC Number   |
|---|
   2.4.99.18 | - |
| 2.4.99.18 | cloning of DNA encoding the full-length AfAglB-S1 gene, expression of N-terminally His6-tagged wild-type and selenomethionine-labeled AglB in Escherichia coli strains BL21(DE3) and C43(DE3), respectively |
| 2.4.99.18 | cloning of OST48, ribophorin I, ribophorin II and expression in COS-1 cells |
| 2.4.99.18 | creation of a yeast strain in which the essential 64000 Da glycoprotein Nlt1p subunit of the oligosaccharyl transferase is modified by the addition of a 22-residue carboxy-terminal affinity tag, the tag includes both an 8-residue FLAG epitope and a 6-residue histidine motif |
| 2.4.99.18 | despite processing distinct lipid-linked glycans in their native hosts, AglB from Haloarcula marismortui can readily replace their counterpart from Haloferax volcanii when introduced into Haloferax volcanii cells deleted of aglB |
| 2.4.99.18 | despite processing distinct lipid-linked glycans in their native hosts, AglB from Halobacterium salinarum can readily replace their counterpart from Haloferax volcanii when introduced into Haloferax volcanii cells deleted of aglB |
| 2.4.99.18 | despite processing distinct lipid-linked glycans in their native hosts, AglB from Haloferax mediterranei can readily replace their counterpart from Haloferax volcanii when introduced into Haloferax volcanii cells deleted of aglB |
| 2.4.99.18 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells |
| 2.4.99.18 | expression in Escherichia coli |
| 2.4.99.18 | functionally expressed in Escherichia coli |
