EC Number |
---|
2.3.1.255 | - |
2.3.1.255 | a plasmid expressing hNaa16p, NARG1L-FLAG, is used |
2.3.1.255 | cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene |
2.3.1.255 | expressed in Escherichia coli BL21 cells |
2.3.1.255 | expressed in Escherichia coli BL21(DE3) cells |
2.3.1.255 | expressed in Escherichia coli BL21(DE3)pLysS cells |
2.3.1.255 | expressed in Escherichia coli Rosetta (DE3)pLysS cells |
2.3.1.255 | expressed in Escherichia coli Rosetta II(DE3)pLysS cells |
2.3.1.255 | expressed in Escherichia coli Rosetta Star cells |
2.3.1.255 | gene NAA10, DNA and amino acid sequence determination and analysis, genotyping, recombinant expression of His-MBP-tagged wild-type and mutant Naa10 in Escherichia coli strain BL21 Star DE3 |