EC Number   |
|---|
    2.3.1.191 | - |
| 2.3.1.191 | by complementation of a temperature-sensitive Escherichia coli lpxD mutant, a meningococcal chromosomal fragment is cloned that carries the lpxD homologue |
| 2.3.1.191 | expression in Escherichia coli BL21(DE3) |
| 2.3.1.191 | gene AtlpxD, two AtLpxD isozymes, AtLxpD1 and AtLpxD2, are encoded on chromosome 4, DNA and amino acid sequence determination and analysis, expression as GFP-tagged protein in Arabidopsis thaliana |
| 2.3.1.191 | gene lpxD, expression as His6-tagged protein in Escherichia coli strain BL21(DE3) |
| 2.3.1.191 | gene lpxD, expression of N-terminally His6-tagged LpxD in Escherichia coli strain BL21 (DE3) |
| 2.3.1.191 | genes lpxD1 and lpxD2, DNA and amino acid sequence determination and analysis, phyylogenetic analysis, the lpxD1gene lies within an essential four-gene operon, lpxD1/fabZ/lpxA/lpxB, while the second gene lpxD2 as a single gene is predicted to be regulated by its own promoter |
| 2.3.1.191 | lpxA (lpxAPg) and lpxDPg are cloned and expressed in Escherichia coli strains in which the homologous gene is mutated. Lipid A from strains expressing either of the Porphyromonas gingivalis transferases contains 16-carbon hydroxy fatty acids in addition to the normal Escherichia coli 14-carbon hydroxy fatty acids, demonstrating that these acyltransferases display a relaxed acyl chain length specificity |
| 2.3.1.191 | LpxD protein modified with an N-terminal His6 tag followed by a one glycine residue linker and the P2A substitution, is constructed and transformed into Escherichia coli Rosetta (DE3)/pLysS |
| 2.3.1.191 | overexpression in Escherichia coli |
