EC Number   |
|---|
   2.3.1.178 | - |
| 2.3.1.178 | cloned into the expression vector pQE30 with a 6×histidine tag and expressed in Escherichia coli JM109 M15 cells |
| 2.3.1.178 | expression in Escherichia coli |
| 2.3.1.178 | expression in Escherichia coli with an additional six His residues at the C-terminus |
| 2.3.1.178 | expression in Escherichia coli, also osmoregulated in Escherichia coli |
| 2.3.1.178 | expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) |
| 2.3.1.178 | gene ectA, ectABC operon, in Marinococcus halophilus, the transcription of ectABC genes is initiated from three individual sigma70/sigmaA-promoters locate upstream of each gene |
| 2.3.1.178 | gene ectA, encoded in ectABC, transcription of the ectABC-ask operon is initiated from two sigma70-dependent promoters ectAp1 and ectAp2. Gene ectR1 encodes the MarR-like transcriptional regulator EctR that is involved in the regulation of ectABC, overview |
| 2.3.1.178 | heterologous production of EctA in Escherichia coli, with either a N-terminal (NH2-WSHPQFEK-SG) or a C-terminal (SA-WSHPQFEK-COOH) Strep-Tag II peptide for their purification by affinity chromatography |
| 2.3.1.178 | into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells |
