EC Number   |
|---|
    2.3.1.15 | - |
| 2.3.1.15 | a full length cDNA of the GPAT gene is subcloned into the expression vector pBI121, the gene is introduced into the tobacco, NC89, by Agrobacterium-mediated leaf disk transformation |
| 2.3.1.15 | chloroplast acyltransferase |
| 2.3.1.15 | cloning of cDNA |
| 2.3.1.15 | DNA and amino acid sequence determination and analysis, full functional complementation by expression of His6-tagged enzyme in an enzyme-deficient yeast mutant strain BYL118, the recombinant enzyme shows a slightly altered substrate specificity, overview |
| 2.3.1.15 | DNA and amino acid sequence determmination and analysis, phylogenetic analysis and tree, quantitative reverse-transcription real-time PCR expression analysis, functional complementation of the osgpat3 mutant, calluses induced from young panicles of the homozygous osgpat3 plants are used for transformation with Agrobacterium tumefaciens strain EHA105 carrying the pCAMBIA1301-OsGPAT3-GFP plasmid or the control plasmid pCAMBIA1301-GFP expressing GFP-tagged wild-type and mutant enzymes, map-based cloning and functional complementation of mutant osgpat3, localization of osgpat3 mutation on chromosome 11 |
| 2.3.1.15 | elimination of isozyme mtGPAT1 in knockout mice |
| 2.3.1.15 | enzyme cloning and overexpression in Phaeodactylum tricornutum strain CCMP2561, PtGPAT cDNA is fused between the fucoxanthin chlorophyll a/c binding protein (fcp) fcpC promoter and fcpA terminator of Phaeodactylum tricornutum. Furthermore, an Omega leader sequence is added before the coding region of PtGPAT for enhancing its translation. A c-Myc tag is also fused to the C-terminal of PtGPAT, quantitative real-time RT-PCR expression analysis |
| 2.3.1.15 | expressed as a myc-epitope-tagged fusion protein in Tobacco suspension cultured cells |
| 2.3.1.15 | expressed in Escherichia coli XL1-Blue cells |
