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EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.183-
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.183Dim1 complements heterologously for ksgA- mutation in Escherichia coli, demonstrating functional equivalence of the two proteins
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.183to delineate regions of the eukaryotic Dim1 critical to its function, KsgA/Dim1 chimeras are created and tested. Construction of six chimeras that result from swapping the two domains of Saccharomyces cerevisiae Dim1, Escherichia coli KsgA, and Methanocaldococcus jannaschii Dim1. Of the chimeras, only one constructed with the N-terminal domain from eukaryotic Dim1 and the C-terminal domain from archaeal Dim1 is able to complement for the eukaryotic Dim1
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