EC Number |
---|
2.1.1.11 | a cDNA library is constructed |
2.1.1.11 | derived from plasmid pRPS404, consisting of a 46 kb pair section of the photosynthetic gene cluster, overexpression of His6-tagged protein in Escherichia coli, strain BL21DE3, expression plasmid pHisBchM |
2.1.1.11 | expressed in Escherichia coli |
2.1.1.11 | expression in Escherichia coli |
2.1.1.11 | expression in Escherichia coli JM109, constitutive promoter on pUCmod, overexpression improved by using BL21 cells in combination with T7 promoter, subclconing into pET-20b(+) vector, engineered Escherichia coli strain overproducing protoporphyrin for activity assays |
2.1.1.11 | expression in Escherichia coli, pCambia derivative, binary vector introduced into the GV3121 strain of Agrobacterium tumefaciens, transgenic plants generated, EYFP fusion protein for localization studies, insertion of the T-DNA in single intron present in the coding sequence, deletion of the 5' part of the gene including the entire first exon, null-mutant |
2.1.1.11 | for sequence determination, mutant complementation |
2.1.1.11 | into the vector pET9a for expression in Escherichia coli BL21DE3 cells |
2.1.1.11 | overexpression in Escherichia coli |