EC Number   |
|---|
   1.8.1.B1 | - |
| 1.8.1.B1 | cloning of two trans-spliced mRNA variants that encode thioredoxin glutathione reductases (mitochondrial and cytosolic selenocysteine-containing isoforms) that possess identical glutaredoxin and thioredoxin reductase domains and differ exclusively in their N-termini |
| 1.8.1.B1 | expresion in Escherichia coli BL21 (DE3) |
| 1.8.1.B1 | expressed in Escherichia coli BL21 (DE3) cells |
| 1.8.1.B1 | expressed in Escherichia coli BL21(DE3) cells |
| 1.8.1.B1 | expression in Escherichia coli |
| 1.8.1.B1 | expression in Escherichia coli as a six-histidine tagged protein. Recombinant His-SmTGR, which would not have a penultimate selenocysteine when expressed in Escherichia coli, has no measurable activity in the insulin reduction assay |
| 1.8.1.B1 | expresssion in Escherichia coli, truncated form without the last two residues Sec597Gly598 |
| 1.8.1.B1 | recombinant enzyme with a fused bacterial-type SECIS element is expressed in Escherichia coli strain BL21(DE3) |
| 1.8.1.B1 | recombinant TGR is mostly insoluble when expressed in Escherichi coli, and neither cloning of the enzyme that contain various tags nor coexpression of TGR with chaperones increases its solubility |
