EC Number   |
|---|
   1.14.18.2 | - |
| 1.14.18.2 | DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotyping and genotype-phenotype correlation analysis, genetic variant c.364C>T is highly associated with blood type AB in Ragdoll cats and, to a lesser degree, with type AB in random bred cats, it is associated with the expression of both antigens (N-glycolylneuraminic acid and N-acetylneuraminic acid) on the red blood cell membrane |
| 1.14.18.2 | DNA and amino acid sequence determination of wild-type and mutant alleles, genotyping and expression analysis, overview |
| 1.14.18.2 | expressed in pig kidney PK15 cells and human vascular endothelial ECV304 cells |
| 1.14.18.2 | expression in Cos-1 cells |
| 1.14.18.2 | expression in Escherichia coli |
| 1.14.18.2 | full-length CMAH cDNA is cloned from umbilical cord blood mononuclear cells and HeLa cells into the pGEM-T easy vector for sequencing, GFP- or FLAG-tagged constructs are prepared using the vectors pFLAG-CMV-2 and pEGFP-C2/pEGFP-C1 |
| 1.14.18.2 | functional recombinant expression of secretory His-tagged enzyme, containing an N-terminal secretion signal peptide, in Spodoptera frugiperda Sf9 cells using the baculovirus infection method, large scale enzyme production |
| 1.14.18.2 | gene CMAH, genotyping, genetic structure, and phylogenetic analysis. Haplotype inference, which is focused on non-synonymous single-nucleotide polymorphisms reveals that eight haplotypes carry one to four mutations in CMAH, and all cats with type B (n = 34) and AB (n = 2) blood carry two alleles derived from the mutated CMAH gene |
| 1.14.18.2 | gene CMAH, recombinant expression in HEK-293T cells |
