EC Number |
---|
1.14.15.1 | ArR/Arx electron-transport chain co-expressed with the CYP enzymes in Escherichia coli. Amplified genes incorporated into the pRSFDuet-Arx vector using the Nde I and Kpn I/EcoR V restriction sites, plasmids pETDuet-Arx-ArR and one of the P450 expressing plasmids pRSFDuet-Arx-CYP transformed into Escherichia coli competent BL21(DE3) cells |
1.14.15.1 | expressed in Escherichia coli strain JM109 |
1.14.15.1 | expression in Escherichia coli |
1.14.15.1 | expression of C334A enzyme mutant in Escherichia coli strain NCM533 |
1.14.15.1 | expression of enzyme and cofactor, separately, in Escherichia coli strain DH5alpha |
1.14.15.1 | expression of enzyme and mutant cofactor in Escherichia coli strain BL21(DE3) |
1.14.15.1 | expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
1.14.15.1 | expression of P450cam C334A in Escherichia coli NCM533 |
1.14.15.1 | expression of wild-type and mutant enzymes in Escherichia coli |
1.14.15.1 | expression of wild-type enzyme, encoded by gene camC, with gene camB, encoding the putidaredoxin, in Escherichia coli strains BL21(DE3), expression of mutant enzymes in Escherichia coli strain DH10B |