EC Number |
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1.1.1.34 | a truncated protein is expressed in Escherichia coli BL21(DE3) cells |
1.1.1.34 | a truncated version of the protein lacking the N-terminal 249-bp GC rich area is expressed in Escherichia coli BL21(DE3) cells |
1.1.1.34 | cDNA encoding the wild-type and mutant forms of human HMG-CoA reductase expressed under control of the yeast MET25 promoter in a Saccharomyces cerevisiae strain with deletions of both HMG1 and HMG2 |
1.1.1.34 | coexpression of the Sulfolobus solfataricus hmgA gene in Escherichia coli with the argU gene that encodes tRNAAGA,AGG results in an over 10fold increase in enzyme yield |
1.1.1.34 | construction of a N-terminally truncated enzyme, comprising the catalytic site, and subcloning into Escherichia coli strain DH5alpha |
1.1.1.34 | DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR and real-time PCR expression analysis |
1.1.1.34 | DNA and amino aid sequence determination and analysis, cloning in Escherichia coli and functional complementation of a HMGR-deficient mutant yeast strain using the yeast-Escherichia coli coli shuttle vector pYF1845 |
1.1.1.34 | expressed in Arabidosis thaliana |
1.1.1.34 | expressed in Escherichia coli |
1.1.1.34 | expressed in Escherichia coli Rosetta (DE3) cells |