EC Number   |
|---|
   1.1.1.331 | - |
| 1.1.1.331 | DNA and amino acid sequence determination and analysis, sequence comparisons, cloning and expression in Escherichiaa coli strain JM109. Confirmation that the SD gene sequence originates from Phialocephala podophylli strain PPE7 fungal gDNA and is not a product from amplification of Podophyllum peltatum gDNA plant contamination is achieved through PCR amplification of any contaminating rbcL plant gene sequences in the PPE7 fungal gDNA template samples |
| 1.1.1.331 | expression in Escherichia coli |
| 1.1.1.331 | functional coexpression of pinoresinol-lariciresinol reductase from Forsythia intermedia (FiPLR), secoisolariciresinol dehydrogenase from Podophyllum pleianthum (PpSDH), and CYP719A23 together with a suitable NADPH-dependent reductase to ensure P450 activity in Escherichia coli. Escherichia coli is cotransformed with two plasmids, generating a modular coexpression system: a pCDFDuet-1 vector harboring the genes encoding for FiPLR and PpSDH (FiPLR-PpSDH module) and either a pETDuet-1 or pET28a-(+) vector harboring P450-redox partner combinations (P450-module), method, overview |
| 1.1.1.331 | gene FkSIRD, DNA and amino acid sequence determination and analysis from Forsythia genome, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis |
| 1.1.1.331 | gene PhSDH, sequence comparisons, semiquantitative RT-PCR expression analysis |
| 1.1.1.331 | gene sdh-PpH, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain M15. Genes plr-PpH, encoding pinoresinol-lariciresinol reductase (PLR), and sdh-PpH are linked to form two bifunctional fusion genes, plr-sdh and sdh-plr, which are expressed as functional His-tagged proteins in Escherichia coli strain M15. Establishment of a high-efficiency system for the conversion of (+)-pinoresinol to (-)-matairesinol in Escherichia coli expressing a Sdh-PpH fusion protein |
| 1.1.1.331 | gene SDH_Pp7, DNA and amino acid sequence determination and analysis, sequence comparisons, cloning and expression in Escherichia coli strain JM109, compatible codon optimization for expression in the heterologous host Pichia pastoris |
| 1.1.1.331 | recombinant overexpression of endogenous enzymes secoisolariciresinol dehydrogenase (SDH) and pinoresinollariciresinol reductase (PLR) in Dysosma versipellis, quantitative RT-PCR expression analysis |
