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EC Number
chemically synthesized gene, mutant Gln58Asp, expressed in Escherichia coli host
expression in Epicurian coli
expression in Escherichia coli
expression in Saccharomyces cerevisiae
the secondary structure unit mutant S2354 is expressed in Escherichia coli only when His102 is substituted by alanine (H102A)
overexpression in Saccharomyces cerevisiae. Characterization of an rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae. The rns4 mutant is sensitive to both RNase T1 and ambient stress. In contrast to the wild-type strain in which the inactivated RNase T1-GFP fusion protein is localized at the vacuole only under cold stress or nitrogen starvation, the inactivated RNase T1-GFP fusion protein expressed in the rns4 mutant is localized at the ER and vacuole, both under normal growth conditions and upon ambient stress conditions
fusion of the antiferritin antibody VL domain to barnase and expression in Escherichia coli
into the pGEM-T vector for transformation of Escherichia coli JM109 cells, into pPIC9K for expression in Pichia pastoris
a 1.8 kb DNA fragment, encoding the C-terminal portion of the MycRne polypeptide, residues 332-953, is cloned into p6HisF-11d for expression in Escherichia coli BL21-CodonPlusDE3-RIL cells
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