EC Number |
Reference |
---|
1.5.5.2 | gene product PutA functions as proline dehydrogenase and as repressor of genes putP and putA |
392555 |
1.5.5.2 | - |
658033, 696361 |
1.5.5.2 | wild-type PutA86-601 and site-directed mutants |
658211 |
1.5.5.2 | expression in Escherichia coli |
658668, 711252 |
1.5.5.2 | gene POX, stable recombinant expression in DLD-1 colorectal cancer cells |
741588 |
1.5.5.2 | gene JcProDH, DNA and amino acid sequence determination and analysis, sequence analysis, phylogenetic analysis, reverse transcription PCR expression analysis, recombinant functional expression in Saccharomyces cerevisiae strain INVSc1 |
741642 |
1.5.5.2 | sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL |
741732 |
1.5.5.2 | gene TT_C1214, recombinant expression of functional and soluble MBP-tagged enzyme in Escherichia coli TOP10 cells. The MBP tag inhibits the self-association of TtProDH, preventing to a large extent the formation of insoluble protein aggregates |
742162 |
1.5.5.2 | gene ProDH, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)plysS |
742641 |
1.5.5.2 | gene Tc00.1047053506411.30, sequence comparisons, recombinant expression of the active His6-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3) cells, reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) expression analysis, functional complementation of ProDH-deficient, PUT1 mutant Saccharomyces cerevisiae strain YLR142W. TcPRODH gene expression in the mutant diminishes free intracellular proline levels and an enhances sensitivity to oxidative stress |
743635 |