EC Number   |
|---|
  3.5.1.60 | recombinant expression of wild-type and mutant enzymes NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide comprising the first 28-33 residues is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Constructs encompassed residues 29-359 of NAAA of human enzyme |
| 3.5.1.60 | recombinant expression of wild-type and mutant enzymes NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Constructs encompass residues 29-355 of NAAA of mouse enzyme |
| 3.5.1.60 | recombinant expression of wild-type and mutant enzymes NAAA in Spodoptera frugiperda Sf9 insect cells via baculovirus transfection method, the enzyme is secreted. The endogenous signal peptide is replaced by the melittin signal peptide MKFLVNVALVFMVVYISYIYA followed by a His6-tag DRHHHHHHKL. Constructs encompass residues 34-362 of NAAA of mouse enzyme |
| 3.5.1.60 | recombinant overexpression in HEK-293 cell membranes |
| 3.5.1.60 | recombinant stable expression of the His6-tagged zymogen in HEK-293 cells, ammonium chloride treatment stimulates secretion of the lysosomal proteins from the HEK293 cells |
