EC Number   |
|---|
   2.7.1.68 | expression of mutant enzymes K181A, D309N, D309N/R429Q and R427Q in Escherichia coli, expression of mutant enzymes in human MG-63 osteosarcoma cells |
| 2.7.1.68 | functional complementation of a yeast Mss4-1 mutant strain YOC808 by the wild-type isozyme gamma and the deletion mutants localizing to the plasma membrane, while the deletion mutants localized to the cytosol cannot support yeast cell growth, expression of wild-type and mutant type I HA-tagged or EGFP-tagged isozymes gamma in Neuro 2A cells and in COS-1 cells |
| 2.7.1.68 | functional expression in HeLa cells as GFP-fusion protein, translocation to the plasma membrane by coexpression of ARF6 upon stimulation by EGF, coexpression of ARF6 mutant N122I inhibits the membrane ruffling, overview |
| 2.7.1.68 | identification of splicing form c of type I phosphatidylinositol 4-phosphate 5-kinase isoform gamma possessing additional 26 amino acids at the C-terminus from a hippocampus library, DNA and amino acid sequence determination and analysis, overvexpression of the catalytically inactive mutants D316K and K188A of isozyme gamma splicing form c in rat cerebellar granule cells, expression of isozyme gamma splicing form a, expression of wild-type and FLAG-tagged isozyme gamma splicing form c in COS-7 cells |
| 2.7.1.68 | isozyme PIP5K1beta expression in HEK-293T and COS-7 cells |
| 2.7.1.68 | overexpression of isozymes alpha, beta, and gamma in HeLa cells, overexpression of isozymes alpha, beta, and gamma in CV-1 cells: isozymes alpha and beta are active, the latter shows the highest activity, isozyme gamma is not active, small interference RNA inhibition of isozyme beta expression also reduces expression of isozyme alpha, but increased expression of isozyme gamma, with a 50% loss in activity, reduction of isozyme alpha expression increases expression of isozymes beta and gamma and leaves the 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate synthesis level unaltered, inhibition of isozyme gamma increases expression of isozymes alpha and beta, overview, expression of isozyme beta increases the internalization of influenza virus hemagglutinin Y543, enzyme expression prevents the cells of disruption of their actine cytoskeleton by drugs, e.g. cytochalasin D or latrunculin, and increases the number of clathrin-coated pits |
| 2.7.1.68 | overexpression of mutant D266A in N1E-115 cells, expression of isozyme alpha in COS-7 cells, expression of isozyme alpha in Escherichia coli |
| 2.7.1.68 | overexpression of PIPKalpha in N1E-115 neuroblastoma cells, co-expression of His6-tagged KIF2A and GST-tagged PIPKalpha in Escherichia coli, binding analysis |
