EC Number   |
|---|
   2.4.1.7 | heterologous expression in a food-grade Bacillus subtilis strain. Efficiently secreted into the extracellular medium in the absence of a signal peptide. After culturing the recombinant strain in a 3-l bioreactor, crude enzyme yield and activity reach 7.5 g/l and 5.3 U/ml, respectively |
| 2.4.1.7 | overexpressed in Escherichia coli, 30% of total soluble protein, high specific activity |
| 2.4.1.7 | recombinnat expression in Escherichia coli |
| 2.4.1.7 | sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 2.4.1.7 | unspase, genetic library construction, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged unspase in Escherichia coli strain XL-1 Blue |
