EC Number   |
|---|
    2.3.1.23 | LPCAT1, DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of FLAG-tagged enzyme in CHO-K1 cells, subcellular distribution pattern analysis shows enzyme expression mainly in the endoplasmic reticulum-Golgi, but also in mitochondria, and lysosome of the transfected cells |
| 2.3.1.23 | LPCAT1, quantitative real-time PCR expression analysis |
| 2.3.1.23 | two genes LPCAT, LPCAT1 and LPCAT2, containing eight exons and seven introns, respectively, DNA and amino acid seqquence determination and analysis, co-expression of flax genes LPCAT1 and diacylglycerol acyltransferase DGAT1-1 in the yeast quintuple mutant strain H1246 DELTAale1 significantly increasing 18-carbon polyunsaturated fatty acids in triacylglycerol with a concomitant decrease of 18-carbon polyunsaturated fatty acids in phospholipid. The specific activity of flax LPCAT1-catalyzed forward reaction is about 1000 times higher than that of flax DGAT1-catalyzed reaction when the expressed recombinant enzymes are assessed in yeast microsomes under in vitro enzyme assays |
