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EC Number	Cloned (Commentary)
1.4.9.1	the methylamine utilization (mau) gene cluster contains 11genes, two of which encode the 42.5 kDa alpha-(mauB) and the 14.2 kDa beta-subunits (mauA) of MADH, recombinant co-overexpression of genes mauAB in Rhodobacter sphaeroides in the presence of mauDEFG, the four genes required for MADH maturation results in an alpha2beta2 MADH protein that has no catalytic activity, because the protein contains all six beta-subunit disulfides, but has a partially synthesized TTQ cofactor with only a single -OH group added to the indole of betaTrp57 (betaTrp57-OH)

EC Number

Cloned (Commentary)

1.4.9.1

the methylamine utilization (mau) gene cluster contains 11genes, two of which encode the 42.5 kDa alpha-(mauB) and the 14.2 kDa beta-subunits (mauA) of MADH, recombinant co-overexpression of genes mauAB in Rhodobacter sphaeroides in the presence of mauDEFG, the four genes required for MADH maturation results in an alpha2beta2 MADH protein that has no catalytic activity, because the protein contains all six beta-subunit disulfides, but has a partially synthesized TTQ cofactor with only a single -OH group added to the indole of betaTrp57 (betaTrp57-OH)

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Release 2023.1 (January 2023)