EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
5.4.99.12 | Escherichia coli tRNAPhe uridine39 |
- |
Escherichia coli |
Escherichia coli tRNAPhe pseudouridine39 |
- |
? |
5.4.99.12 | human tRNALys3 |
- |
Mus musculus |
? |
- |
? |
5.4.99.12 | human tRNASer uridine39 |
- |
Mus musculus |
human tRNASer pseudouridine39 |
- |
? |
5.4.99.12 | more |
presence of a G36 residue modulates modification at position 38. In addition to local sequence effects, steady-state kinetic analyses suggest the existence of other recognition elements distinct from the immediate vicinity of modification |
Escherichia coli |
? |
- |
? |
5.4.99.12 | Salmonella typhimurium HisT- tRNALeu |
tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs |
Bos taurus |
? |
- |
? |
5.4.99.12 | Salmonelly typhimurium HisT- tRNATyr |
tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs |
Bos taurus |
? |
- |
? |
5.4.99.12 | tRNA uridine38 |
- |
Saccharomyces cerevisiae |
tRNA pseudouridine38 |
- |
? |
5.4.99.12 | tRNA uridine38 |
tRNALeu3 contains uridine at position 38. Wild-typeTruA pseudouridylates uridines at all three positions (38, 39 and 40) with efficiencies (kcat/KM) differing by less than 10fold, while R58A is inactive toward all three uridines. When flexibility of the anticodon stem loop is increased by mutating the two G:C base pairs in the stem of the anticodon stem loop of tRNALeu3 into A:U pairs, the kcat/KM increased 2fold. When flexibility is decreased by base-pairing the target U38 of tRNALeu3 with A32 instead of with U32, the kcat/KM decreases 10fold |
Escherichia coli |
tRNA pseudouridine38 |
- |
? |
5.4.99.12 | tRNA uridine38 |
the enzyme modifies the anticodon arm of transfer RNA at positions 38 and 39 by catalyzing the conversion of uridine to pseudouridine |
Saccharomyces cerevisiae |
tRNA pseudouridine38 |
- |
? |
5.4.99.12 | tRNA uridine38-40 |
- |
Thermus thermophilus |
tRNA pseudouridine38-40 |
- |
? |