EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
- |
Streptomyces ravidus |
? |
- |
? |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
- |
Streptomyces ravidus |
? |
no characterization of the product due to its low yield |
? |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
- |
Acinetobacter baumannii |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
- |
? |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway |
Aneurinibacillus thermoaerophilus |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose |
r |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
the enzyme is involved in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose. Four moieties of alpha-D-rhamnose and two moities of 3-acetamido-3,6-dideoxy-alpha-D-galactose form the repeating unit of the glycan chain in the S-layer of the bacterium Aneurinibacillus thermoaerophilus |
Aneurinibacillus thermoaerophilus |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
- |
? |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The activity of FdtA is monitored by a simultaneous incubation with dTDP-6-deoxy-D-xylohex-4-ulose with FdtB and the required cosubstrates. The resulting product, dTDP-D-Fucp3N, is detectable by HPLC analysis |
Aneurinibacillus thermoaerophilus |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose |
r |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
Site-directed mutagenesis experiments, enzymatic assays, and X-ray crystallographic analyses suggest that His49 functions as an active site base |
Aneurinibacillus thermoaerophilus |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
- |
? |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
- |
Acinetobacter baumannii RCH51 |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
- |
? |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway |
Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose |
r |
| 5.3.2.3 | dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The activity of FdtA is monitored by a simultaneous incubation with dTDP-6-deoxy-D-xylohex-4-ulose with FdtB and the required cosubstrates. The resulting product, dTDP-D-Fucp3N, is detectable by HPLC analysis |
Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose |
i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose |
r |
