EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   4.2.99.B1 | 25-mer-deoxyoligonucleotide 3'-labeled uracil-containing DNA duplex |
[32P]-labeled substarte, treated with uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease |
Mus musculus |
? + 2-deoxy-D-ribose 5-phosphate |
the products are stabilized by NaBH4 reduction of the Schiff base, formed between the catalytic nucleophile of the dRP lyase and C1' of rhe 2-deoxyribose 5-phosphate site, verifying the dRP removal by a beta-elimination |
? |
| 4.2.99.B1 | 28-mer deoxyoligonucleotide with a 5' uracil residue |
pretreated with Eschericia coli uracil N-glycosylase |
Crithidia fasciculata |
? |
- |
? |
| 4.2.99.B1 | 34-bp oligonucleotide containing uracil at position 16 |
labelled with 3'-end by terminal deoxynucleotidyltransferase using [alpha-32P]ddATP and annealed to its complementary oligonucleotide, and pretreated with human uracil-DNA glycosylase and AP endonuclease |
Homo sapiens |
? + 2-deoxy-D-ribose 5-phosphate |
- |
? |
| 4.2.99.B1 | 34-bp-deoxyoligonucleotide duplex containing a uracil residue at position 16 |
preincised [32P] apurinic/apyrimidinic site containing DNA, pretreated with uracil DNA-glycosylase and AP endonuclease |
Homo sapiens |
? + 2-deoxy-D-ribose phosphate |
the reaction products are separarted by electrophoresis |
? |
| 4.2.99.B1 | 34-bp-oligonucleotide containing uracil at position 16 |
labelled with 3'-end by terminal deoxynucleotidyltransferase using [alpha-32P]ddATP and annealed to its complementary oligonucleotide, and pretreated with human uracil-DNA glycosylase and AP endonuclease |
Leishmania infantum |
19-bp-oligonucleotide + 2-deoxy-D-ribose 5-phosphate |
- |
? |
| 4.2.99.B1 | 34-mer-deoxyoligonucleotide containing a uracil residue at position 16 |
pretreated with uracil-N-glycosylase and apurunic/apyrimidinic endonuclease |
Trypanosoma cruzi |
18-mer-deoxyoligonucleotide + 2-deoxy-D-ribose 5-phosphate |
- |
? |
| 4.2.99.B1 | 35-bp-deoxyoligonucleotide duplex containing a uracil residue at position 15 |
in vitro single-nucleotide base excision DNA repair assay, containing amongst others uracil DNA-glycosylase, AP endonuclease and ligase I |
Homo sapiens |
? + 2-deoxy-D-ribose phosphate |
- |
? |
| 4.2.99.B1 | 49-bp oligodeoxynucleotide containing uracil at position 21 |
labelled at 3'-end with [alpha-32P]ddATP by terminal deoxynucleotidyl transferase and annealed to an unlabelled complementary strand containing a G residue opposite the uracil. Before use, the substrate is treated with uracil-DNA glycosylase and AP endonuclease to generate a single nucleotide gap directly upstream from the labelled fragment containing a deoxyribose phosphate flap |
Homo sapiens |
? + 2-deoxy-D-ribose 5-phosphate |
- |
? |
| 4.2.99.B1 | 49-residue oligonucleotide duplex DNA |
substrate contains uracil residue at position 21. The DNA is pretreated with uracil DNA glycosylase and AP endonuclease |
Homo sapiens |
? + 2-deoxy-D-ribose 5-phosphate |
- |
? |
| 4.2.99.B1 | 5'-deoxyribose phosphate DNA substrate |
- |
Homo sapiens |
? |
- |
? |
