EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
- |
Pseudomonas fluorescens |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
- |
Pseudomonas sp. |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
- |
Pseudomonas brassicacearum |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
the enzyme functions as a hydrolase specifically degrades 2,4-diacetylphloroglucinol, DAPG, to equimolar amounts of mildly toxic monoacetylphloroglucinol (MAPG) and acetate |
Pseudomonas fluorescens |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
2,4-diacetylphloroglucinol hydrolase catalyzes hydrolytic C-C bond cleavage, cleaving one of the C-C bonds linking the acetyl groups to the phenolic ring, of the antibiotic 2,4-diacetylphloroglucinol to form monoacetylphloroglucinol, a rare class of reactions in chemistry and biochemistry, strict substrate specificity, no degradation of other compounds of similar structure such as monoacetylphloroglucinol and triacetylphloroglucinol |
Pseudomonas fluorescens |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
modeling of a theoretical transition state for the substrate hydrolysis into the active site revealing that a water molecule, bound by the zinc, can be responsible for initiating the C-C bond cleavage reaction through nucleophilic attack at the acyl carbonyl carbon atom. Residue Asn132 in Phlg is thought to make a hydrogen bond to the acyl group of the native substrate in the enzyme. Tyr121 is thought to hydrogen bond to the phenol group in the 1-position of the aromatic ring between the two acetyl groups in the substrate |
Pseudomonas fluorescens |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
degradation of 2,4-diacetylphloroglucinol catalyzed by PhlG plays an insignificant role in 2,4-diacetylphloroglucinol tolerance but contributes to bacterial growth advantages under carbon/nitrogen starvation conditions |
Pseudomonas fluorescens |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
2,4-diacetylphloroglucinol i.e. 1,1'-(2,4,6-trihydroxybenzene-1,3-diyl)diethan-1-one |
Pseudomonas brassicacearum |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
- |
Pseudomonas fluorescens LRB3W1 |
2-acetylphloroglucinol + acetate |
- |
? |
| 3.7.1.24 | 2,4-diacetylphloroglucinol + H2O |
- |
Pseudomonas fluorescens Pf-5 |
2-acetylphloroglucinol + acetate |
- |
? |
