EC Number   |
Substrates |
Organism |
Products |
Reversibility  |
|---|
   3.4.24.11 | more |
activity measurement optimization using the synthetic fluorogenic peptide substrates, evaluation and kinetics, overview |
Homo sapiens |
? |
- |
- |
| 3.4.24.11 | more |
analysis of intact amyloid-beta peptides and their NEP cleavage products by mass spectrometry. 73% of amyloid-beta4-40, 31% of amyloid-beta4-42, 45% of amyloid-beta1-40, and 18% of amyloid-beta1-42 degradation resulting from NEP catalyzed hydrolysis. Amyloid-beta4-9/amyloid-beta1-9, amyloid-beta10-17, and amyloid-beta20-30 are the major enzymatic products |
Homo sapiens |
? |
- |
- |
| 3.4.24.11 | more |
in vitro NEP proteolysis generates fragments that lack the ability to bind to the apelin receptor, NEP fully inactivates apelin |
Homo sapiens |
? |
- |
- |
| 3.4.24.11 | more |
murine amyloid-beta has a far lower propensity to aggregate than human amyloid-beta. Amyloid-beta degradation products formed during NEP incubation are identified by mass spectrometry, analysis of predominant NEP cleavage sites detected by mass spectrometry for human and murine amyloid-beta1-42 and amyloid-beta.4-15. In addition to the amyloid-beta4-9 fragment, di- and tetra-peptides amyloid-beta18-19 and amyloid-beta39-42 are also observed |
Homo sapiens |
? |
- |
- |
| 3.4.24.11 | more |
NEP degrades murine amyloid-beta faster than human amyloid-beta, observed with full-length amyloid-beta containing 40 or 42 amino acids (Abeta1-40 and Abeta1-42) and a truncated form (Abeta4-15), which contains one of the main NEP cleavage sites for amyloid-beta (between positions 9 and 10), harbours all three amino acid differences between murine and human amyloid-beta sequences, and is less prone to aggregation and thus might be a simpler model to investigate amyloid-beta biochemistry. Murine amyloid-beta has a far lower propensity to aggregate than human amyloid-beta. Amyloid-beta degradation products formed during NEP incubation are identified by mass spectrometry, analysis of predominant NEP cleavage sites detected by mass spectrometry for human and murine amyloid-beta1-42 and amyloid-beta.4-15. In addition to the amyloid-beta4-9 fragment, di- and tetra-peptides amyloid-beta18-19 and amyloid-beta39-42 are also observed |
Mus musculus |
? |
- |
- |
| 3.4.24.11 | (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(2,4-dinitrophenyl) + H2O |
a bradykinin-based peptide substrate QFS |
Homo sapiens |
? |
- |
? |
| 3.4.24.11 | (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(2,4-dinitrophenyl) + H2O |
bradykinin-based quenched fluorescent substrate assay |
Homo sapiens |
(7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala + Phe-Lys-(2,4-dinitrophenyl) |
- |
? |
| 3.4.24.11 | adrenocorticotropic hormone + H2O |
- |
Lactococcus lactis |
? |
- |
? |
| 3.4.24.11 | adrenomedullin + H2O |
- |
Homo sapiens |
? |
- |
? |
| 3.4.24.11 | Ala-Leu-enkephalin + H2O |
- |
Oryctolagus cuniculus |
? |
- |
? |
