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EC Number	Substrates	Commentary Substrates	Organism	Products	Commentary (Products)	Reversibility
3.4.23.43	GspG prepseudopilin + H2O	-	Acinetobacter nosocomialis	GspG pseudopilin + GspG prepseudopilin leader peptide	-	?
3.4.23.43	GspG prepseudopilin + H2O	-	Acinetobacter nosocomialis M2	GspG pseudopilin + GspG prepseudopilin leader peptide	-	?
3.4.23.43	Flp1 prepilin + H2O	-	Aggregatibacter actinomycetemcomitans	Flp1 pilin + Flp1 prepilin leader peptide	-	?
3.4.23.43	Flp1 prepilin + H2O	the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pseudopilins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms	Aggregatibacter actinomycetemcomitans	Flp1 pilin + Flp1 prepilin leader peptide	-	?
3.4.23.43	more	activity rate with different recombinant mutants of Flp1, TadE, and TadF constructed by site-directed mutagenesis and expressed in Escherichia coli, overview	Aggregatibacter actinomycetemcomitans	?	-	?
3.4.23.43	TadE prepseudopilin + H2O	-	Aggregatibacter actinomycetemcomitans	TadE pseudopilin + TadE prepseudopilin leader peptide	-	?
3.4.23.43	TadE prepseudopilin + H2O	the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms	Aggregatibacter actinomycetemcomitans	TadE pseudopilin + TadE prepseudopilin leader peptide	-	?
3.4.23.43	TadF prepseudopilin + H2O	-	Aggregatibacter actinomycetemcomitans	TadF pseudopilin + TadF prepseudopilin leader peptide	-	?
3.4.23.43	TadF prepseudopilin + H2O	the enzyme is required for maturation of the Flp1 pilin and TadE and TadF pilin-like proteins which are essential for adhesion of the bacterium to surfaces and formation of strong biofilms	Aggregatibacter actinomycetemcomitans	TadF pseudopilin + TadF prepseudopilin leader peptide	-	?
3.4.23.43	prepilin type IV + H2O	-	Bacillus subtilis	pilin type IV + leader peptide	-	?

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Release 2023.1 (January 2023)