EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.4.21.86 | benzyloxycarbonyl-Leu-Gly-Arg-4-aminophenyl ester + H2O |
- |
Limulus polyphemus |
benzyloxycarbonyl-Leu-Gly-Arg + 4-aminophenol |
- |
? |
3.4.21.86 | Coagulogen + H2O |
- |
Limulus sp. |
Coagulin + fragments |
- |
? |
3.4.21.86 | coagulogen + H2O |
- |
Limulus polyphemus |
coagulin + propeptide |
- |
? |
3.4.21.86 | coagulogen + H2O |
chromogenic substrate |
Limulus polyphemus |
coagulin + propeptide |
- |
? |
3.4.21.86 | Limulus amebocyte lysate + H2O |
bacterial endotoxins induce the formation of small particles of clotted enzyme |
Limulus polyphemus |
small particles of clotted enzyme |
- |
? |
3.4.21.86 | more |
the clotting enzyme can be used in the Limulus amebocytelysate (LAL) assay. The kinetic chromogenic LAL assay uses a synthetic peptide-4-nitroanilide substrate that is cleaved by the clotting enzyme, resulting in a product that exhibits a yellow colour. The intensity of the yellow colour or the rate of colour formation correlates with the concentration of LPS in the assayed samples. The coagulation cascade of the LAL system can also be activated via activation of factor G by fungal glucans, although the activation of factor C, which then activates factor B, by LPS is many times more sensitive. Assay method development and evaluation, overview |
Limulus polyphemus |
? |
- |
? |
3.4.21.86 | peptide-4-nitroanilide + H2O |
- |
Limulus polyphemus |
peptide + 4-nitroaniline |
- |
? |