EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
- |
Bacillus subtilis |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis |
Bacillus subtilis |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
- |
Bacillus subtilis 168 |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis |
Bacillus subtilis 168 |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
- |
Bacillus subtilis |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
- |
Clostridium acetobutylicum |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
postulation of the enzyme reaction mechanism: YteR triggers the hydration of vinyl ether group in 4-deoxy-beta-L-threo-hex-4-enopyranuronose (DELTAGalA), but not of glycoside bond, by using Asp143 as a general acid and base catalyst. Asp143 donates proton to the double bond of 4-deoxy-beta-L-threo-hex-4-enopyranuronose as an acid catalyst and also deprotonates a water molecule as a base catalyst. Deprotonated water molecule attacks the C5 atom of 4-deoxy-beta-L-threo-hex-4-enopyranuronose |
Bacillus subtilis |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
postulation of the enzyme reaction mechanism: YteR triggers the hydration of vinyl ether group in 4-deoxy-beta-L-threo-hex-4-enopyranuronose (DELTAGalA), but not of glycoside bond, by using Asp143 as a general acid and base catalyst. Asp143 donates proton to the double bond of 4-deoxy-beta-L-threo-hex-4-enopyranuronose as an acid catalyst and also deprotonates a water molecule as a base catalyst. Deprotonated water molecule attacks the C5 atom of 4-deoxy-beta-L-threo-hex-4-enopyranuronose |
Bacillus subtilis 168 |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
- |
Bacillus subtilis 168 |
4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose |
- |
? |
3.2.1.172 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O |
- |
Bacillus subtilis |
5-dehydro-4-deoxy-D-glucuronate + L-rhamnopyranose |
- |
? |