EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.1.22.5 | linear double-stranded DNA + H2O |
little activity |
Escherichia coli |
? |
- |
? |
3.1.22.5 | linear double-stranded DNA + H2O |
little activity |
Escherichia coli B / ATCC 11303 |
? |
- |
? |
3.1.22.5 | supercoiled plasma DNA + H2O |
- |
Escherichia coli |
linear DNA duplexes |
- |
? |
3.1.22.5 | supercoiled plasma DNA + H2O |
enzyme recognizes cruziforme structure in supercoiled plasmid DNA, e.g. pBR322 form I DNA is first converted to relaxed circular form II-dimer and subsequently to linear form III-dimer of pBR322, ColE-1 DNA is converted to linear products, the enzyme appears to cleave at more than one site on superhelical pBR322 DNA, a double-strand break at one site seems to block further double-strand cleavage in the DNA molecule |
Escherichia coli |
linear DNA duplexes |
- |
? |
3.1.22.5 | supercoiled plasma DNA + H2O |
not: lambda DNA |
Escherichia coli |
linear DNA duplexes |
- |
? |
3.1.22.5 | supercoiled plasma DNA + H2O |
- |
Escherichia coli B / ATCC 11303 |
linear DNA duplexes |
- |
? |
3.1.22.5 | supercoiled plasma DNA + H2O |
enzyme recognizes cruziforme structure in supercoiled plasmid DNA, e.g. pBR322 form I DNA is first converted to relaxed circular form II-dimer and subsequently to linear form III-dimer of pBR322, ColE-1 DNA is converted to linear products, the enzyme appears to cleave at more than one site on superhelical pBR322 DNA, a double-strand break at one site seems to block further double-strand cleavage in the DNA molecule |
Escherichia coli B / ATCC 11303 |
linear DNA duplexes |
- |
? |
3.1.22.5 | supercoiled plasma DNA + H2O |
not: lambda DNA |
Escherichia coli B / ATCC 11303 |
linear DNA duplexes |
- |
? |