EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.8.3.B1 | 4-methylpent-2-enoyl-CoA + (R)-2-hydroxy-4-methylpentanoate |
the enzyme is involved in the reductive branch of L-leucine fermentation |
Clostridioides difficile |
4-methylpent-2-enoate + (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
? |
2.8.3.B1 | 4-methylpent-2-enoyl-CoA + (R)-2-hydroxy-4-methylpentanoate |
i.e. isocaprenoyl-CoA. Acetyl-CoA or butyryl-CoA, are not accepted as substrates. The enzymatically in situ-produced 2-isocaprenoyl-CoA is accepted as a substrate of the CoA transferase and mediates the tranfer to (R)-2-hydroxy-4-methylpentanoate. CoA transfers from chemically synthesized (E)-2-isocaprenoyl-CoA to (R)-2-hydroxyisocaproate or to isocaproate can not be observed |
Clostridioides difficile |
4-methylpent-2-enoate + (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
r |
2.8.3.B1 | 4-methylpent-2-enoyl-CoA + (R)-2-hydroxy-4-methylpentanoate |
the enzyme is involved in the reductive branch of L-leucine fermentation |
Clostridioides difficile DSM 1296T |
4-methylpent-2-enoate + (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
? |
2.8.3.B1 | 4-methylpent-2-enoyl-CoA + (R)-2-hydroxy-4-methylpentanoate |
i.e. isocaprenoyl-CoA. Acetyl-CoA or butyryl-CoA, are not accepted as substrates. The enzymatically in situ-produced 2-isocaprenoyl-CoA is accepted as a substrate of the CoA transferase and mediates the tranfer to (R)-2-hydroxy-4-methylpentanoate. CoA transfers from chemically synthesized (E)-2-isocaprenoyl-CoA to (R)-2-hydroxyisocaproate or to isocaproate can not be observed |
Clostridioides difficile DSM 1296T |
4-methylpent-2-enoate + (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
r |
2.8.3.B1 | 4-methylpentanoyl-CoA + (R)-2-hydroxy-4-methylpentanoate |
i.e. isocaproyl-CoA. Acetyl-CoA or butyryl-CoA, are not accepted as substrates. CoA transfers from chemically synthesized (E)-2-isocaprenoyl-CoA to (R)-2-hydroxyisocaproate or to isocaproate can not be observed |
Clostridioides difficile |
4-methylpentanoate + (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
r |
2.8.3.B1 | 4-methylpentanoyl-CoA + (R)-2-hydroxy-4-methylpentanoate |
i.e. isocaproyl-CoA. Acetyl-CoA or butyryl-CoA, are not accepted as substrates. CoA transfers from chemically synthesized (E)-2-isocaprenoyl-CoA to (R)-2-hydroxyisocaproate or to isocaproate can not be observed |
Clostridioides difficile DSM 1296T |
4-methylpentanoate + (R)-2-hydroxy-4-methylpentanoyl-CoA |
- |
r |