EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.4.1.269 | GDP-alpha-D-glucose + D-glycerate |
- |
Selaginella moellendorffii |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP |
- |
? |
| 2.4.1.269 | GDP-alpha-D-glucose + D-glycerate |
the enzyme catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate |
Rhodothermus marinus |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP |
- |
? |
| 2.4.1.269 | GDP-alpha-D-glucose + D-glycerate |
at 37°C mannosylglycerate synthase shows donor flexibility and can use alpha-GDP-D-Man, alpha-GDP-D-Glc, beta-GDP-L-fucose, alpha-UDP-D-Man and alpha-UDP-D-Glc, but not Man-1-P, as substrates. The kcat/KM-value for GDP-glucose is 2.5fold lower than the value for GDP-mannose at 25°C. The kcat/KM-value for GDP-glucose is 8.6fold lower than the value for GDP-mannose at 65°C |
Rhodothermus marinus |
2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP |
- |
? |
| 2.4.1.269 | GDP-mannose + D-glycerate |
- |
Selaginella moellendorffii |
2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP |
- |
? |
| 2.4.1.269 | GDP-mannose + D-glycerate |
- |
Rhodothermus marinus |
2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP |
- |
? |
| 2.4.1.269 | GDP-mannose + D-glycerate |
Rhodothermus marinus possesses two enzymatic systems for the synthesis of mannosylglycerate. The first one is a single-step pathway in which mannosylglycerate synthase catalyses the synthesis of 2-O-(alpha-D-mannopyranosyl)-D-glycerate in one-step from GDP-mannose and D-glycerate. The second system is a two-step pathway in which mannosyl-3-phosphoglycerate synthase (EC 2.4.1.217) catalyses the conversion of GDP-mannose and 3-phospho-D-glycerate into 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate, which is then converted to 2-O-(alpha-D-mannopyranosyl)-D-glycerate by mannosyl-3-phosphoglycerate phosphatase (EC 3.1.3.70) |
Rhodothermus marinus |
2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP |
- |
? |
| 2.4.1.269 | GDP-mannose + D-glycerate |
at 37°C mannosylglycerate synthase shows donor flexibility and can use alpha-GDP-D-Man, alpha-GDP-D-Glc, beta-GDP-L-fucose, alpha-UDP-D-Man and alpha-UDP-D-Glc, but not Man-1-P, as substrates. The kcat/KM-value for GDP-glucose is 2.5fold lower than the value for GDP-mannose at 25°C. The kcat/KM-value for GDP-glucose is 8.6fold lower than the value for GDP-mannose at 65°C |
Rhodothermus marinus |
2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP |
- |
? |
| 2.4.1.269 | GDP-mannose + D-glycerate |
the enzyme is specific for GDP-mannose and D-glycerate. The enzyme shows no activity for the reverse reaction. No activity with: ADP-mannose, UDP-mannose (less than 3% of that of GDP mannose), GDP-glucose, UDP-glucose, ADP-glucose, mannose 1-phosphate, and mannose 6-phosphate as sugar donors, and L-glycerate, glycerol 3-phosphate, glucose 6-phosphate, glucose 1-phosphate and D-3-phosphoglycerate as sugar acceptors |
Rhodothermus marinus |
2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP |
- |
ir |
| 2.4.1.269 | GDP-mannose + D-lactate |
- |
Rhodothermus marinus |
? |
- |
? |
| 2.4.1.269 | GDP-mannose + D-lactate |
the kcat/KM-value for D-lactate is 70fold lower than the value for D-glycerate at 25°C |
Rhodothermus marinus |
? |
- |
? |
