EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.252 | GDP-mannose + cellobiose-diphosphate-polyprenol carrier lipid |
GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum |
Xylella fastidiosa |
GDP + D-mannose-alpha-(1-3)-cellobiose-diphosphate-polyprenol carrier lipid |
- |
? |
2.4.1.252 | GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
- |
Komagataeibacter xylinus |
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
- |
? |
2.4.1.252 | GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
the enzyme is involved in the biosynthesis of the exopolysaccharide acetan |
Komagataeibacter xylinus |
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
- |
? |
2.4.1.252 | GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity |
Komagataeibacter xylinus |
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
characterisation of the in vitro reaction product |
? |
2.4.1.252 | GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
EDTA-treated Xanthomonas campestris cells are used as both enzyme preparation and lipid-P acceptor, and UDP-Glc, GDP-Man, and UDP-glucuronic acid are used as sugar donors. A linear pentasaccharide unit is assembled on a polyprenol-phsophate lipid carrier by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose |
Xanthomonas campestris |
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol |
- |
? |
2.4.1.252 | GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphophytanol |
synthetic phytanyl-pyrophosphate-linked cellobiose functions as an acceptor for the AceA mannosyltransferase reaction in vitro. Neither the chain length nor the saturation state, notably of the first isoprene unit, are critical to the AceA mannosyltransferase activity |
Komagataeibacter xylinus |
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphophytanol |
- |
? |
2.4.1.252 | GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol |
- |
Xanthomonas campestris |
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol |
- |
? |
2.4.1.252 | GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol |
the enzyme is involved in biosynthesis of xanthan |
Xanthomonas campestris |
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol |
- |
? |
2.4.1.252 | more |
AceA Lys211 and Glu295 are involved in nucleotide sugar donor binding. Glu287 of the EX7E is a potential catalytic residue |
Komagataeibacter xylinus |
? |
- |
? |
2.4.1.252 | more |
GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively |
Xanthomonas oryzae |
more |
- |
? |